Preparation of engineered metal ion nanovesicles and their applications in cancer immunotherapy(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2026年第4期
- Page:
- 510-518
- Research Field:
- 生物材料与力学
- Publishing date:
Info
- Title:
- Preparation of engineered metal ion nanovesicles and their applications in cancer immunotherapy
- Author(s):
- KONG Jing1; 2; PAN Yuanwei2; ZHANG Wenbing1
- 1. School of Physics and Technology, Wuhan University, Wuhan 430072, China 2. Institute of Chemical Biology, Shenzhen Bay Laboratory, Shenzhen 518132, China
- Keywords:
- Keywords: breast cancer engineered nanovesicle zinc ion tumor immunotherapy
- PACS:
- R318
- DOI:
- DOI:10.3969/j.issn.1005-202X.2026.04.014
- Abstract:
- Abstract: Objective To prepare engineered nanovesicles loaded with zinc ions (Zn-CM@PD1-NVs) and investigate their immunotherapeutic efficacy against murine breast cancer. Methods The 4T1-PD1 cell membrane was complexed with zinc ions to form zinc-loaded membrane fragments (Zn-CM) which was then mixed with a cell membrane suspension, followed by vortexing, sonication, and extrusion through polycarbonate membranes to prepare Zn-CM@PD1-NVs. High PD1 expression was confirmed by flow cytometry and immunofluorescence. The physicochemical properties were characterized using dynamic light scattering, transmission electron microscopy, and inductively coupled plasma optical emission spectroscopy. Biocompatibility was evaluated through CCK-8 assay, hemolysis test, immunofluorescence, blood biochemistry, complete blood count, and H&E staining. A 4T1 subcutaneous tumor model was developed in BALB/c mice, and 3 groups, namely control, PD1-NVs, and Zn-CM@PD1-NVs groups, were established to assess the antitumor efficacy. Additionally, tumor microenvironment immune cells were analyzed by flow cytometry. Results (1) Zn-CM@PD1-NVs exhibited uniform and stable particle size with a core-shell structure. (2) In vitro experiments showed no significant cytotoxicity. (3) No abnormalities were observed in hepatic and renal function indicators, complete blood count measures, or histopathological sections. (4) Zn-CM@PD1-NVs group significantly inhibited 4T1 tumor growth, and increased the infiltration of CD8?T cells in the tumor microenvironment. Conclusion Zn-CM@PD1-NVs is successfully prepared, demonstrating not only excellent biological stability and biocompatibility, but also effective inhibition of breast cancer growth and promotion of antitumor immunity.
Last Update: 2026-04-29