Preparation, drug loading, and immune activation of genetically engineered bacterial vesicles(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2025年第6期
- Page:
- 825-831
- Research Field:
- 生物材料与力学
- Publishing date:
Info
- Title:
- Preparation, drug loading, and immune activation of genetically engineered bacterial vesicles
- Author(s):
- TANG Yuji1; 2; TANG Jiazhen1; 2; RAO Lang2; WEI Yahong1
- 1. College of Life Sciences, Northwest A&F University, Yangling 712100, China; 2. Institute of Chemical Biology, Shenzhen BayLaboratory, Shenzhen 518132, China
- Keywords:
- gene modification; interferon α; bacterial vesicle; drug delivery; photodynamic therapy
- PACS:
- R318.08；R392
- DOI:
- DOI:10.3969/j.issn.1005-202X.2025.06.018
- Abstract:
- Objective To investigate the antitumor effect of genetically engineered bacterial vesicles loaded with thephotosensitizer Ce6. Methods Genetic engineering techniques were used to overexpress interferon α (IFNα) in Escherichiacoli Nissle 1917 (EcN), and the corresponding bacterial vesicles (BVs-IFNα) were extracted. The photosensitizer Ce6 wasloaded onto the surface of the bacterial vesicles via ultrasonication to obtain BVs-IFNα@Ce6 nanoparticles. The BVs-IFNα@Ce6complex was characterized using dynamic light scattering, nanoparticle tracking technology, confocal imaging,and ultraviolet absorption spectroscopy. The obtained BVs-IFNα@Ce6 nanoparticles were co-cultured with mouse melanomacells (B16F10) and mouse bone marrow-derived dendritic cells (BMDC). The efficacy of photodynamic therapy wasassessed using the CCK-8 method, and its ability to induce immune activation was verified using flow cytometry. ResultsThe BVs-IFNα@Ce6 nanoparticles were successfully prepared with a stable particle size of around 180 nm. Under red lightirradiation, BVs-IFNα@Ce6 exhibited significant cytotoxicity against B16F10 tumor cells and effectively promoted theexpressions of immune-related molecules MHC-II, CD80/CD86 on the surface of BMDC cells, demonstrating its remarkableability to induce immune activation. Conclusion The BVs-IFNα@Ce6 nanoparticles possess dual functions of enhancingphotodynamic therapy efficacy and activating antitumor immunity, showing potential for clinical translation.
Last Update: 2025-07-01