Effects of electrical field stimulation on the proliferation and migration of Schwann cells(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2025年第2期
- Page:
- 240-244
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Effects of electrical field stimulation on the proliferation and migration of Schwann cells
- Author(s):
- QI Jingtian1; YE Yongping1; XU Yongjun2; SHENG Qingsong3; CAI Longyu1; HU Jianwei1; ZHANG Yongguang1
- 1. Department of Orthopaedics, 900 Hospital of the Joint Logistics Team, Fuzong Clinical Medical College of Fujian Medical University/Dongfang Affiliated Hospital of Xiamen University, Fuzhou 350025, China 2. Fujian Provincial Key Laboratory of Transplant Biology, 900 Hospital of the Joint Logistics Team, Fuzong Clinical Medical College of Fujian Medical University/Dongfang Affiliated Hospital of Xiamen University, Fuzhou 350025, China 3. Department of Obstetrics and Gynecology, 900 Hospital of the Joint Logistics Team, Fuzong Clinical Medical College of Fujian Medical University/Dongfang Affiliated Hospital of Xiamen University, Fuzhou 350025, China
- Keywords:
- Keywords: electrical field stimulation electrotaxis chamber Schwann cell migration proliferation epithelial-mesenchymal transition
- PACS:
- R35
- DOI:
- DOI:10.3969/j.issn.1005-202X.2025.02.015
- Abstract:
- Objective To establish an electrical field (EF) stimulation model for Schwann cells (SCs), and to provide a basis for exploring the mechanisms of EF stimulation in promoting proliferation, migration and epithelial-to-mesenchymal transition of SCs. Methods A YC-3 bipolar programmable electrical stimulator and an electrotaxis chamber were used to construct an EF stimulation system to stimulate SCs. In the study, SCs were divided into control group (Ctrl) receiving no EF stimulation and EF group stimulated by continuous constant-voltage EF (100 mV/mm, 3 h). The effects of EF stimulation on the proliferation and migration of SCs were analyzed using CCK-8 assay, and wound healing assay + Transwell assay, separately and its effect on SCs adhesion was observed by analyzing the expressions of E-cadherin and N-cadherin using Western Blot. Results The CCK-8 assay results suggested that the absorbance at 450 nm was significantly higher in EF group than in Ctrl group (P<0.05). The results of wound healing assay + Transwell assay revealed that EF group had higher cell migration efficiency than Ctrl group (P<0.05). Western Blot results showed decreased E-cadherin expression and increased N-cadherin expression in EF group as compared with Ctrl group (P<0.05). Conclusion The improved EF stimulation system for SCs is operable. EF stimulation can promote the proliferation and migration of SCs. The decreased E-cadherin expression and increased N-cadherin expression may be related to the occurrence of epithelial-to-mesenchymal transition in SCs after EF stimulation.
Last Update: 2025-01-22