Abstract

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Title:: Role of Ca2+ in electromagnetic field regulation on osteoblast proliferation and differentiation

Author(s):: ZHANG Guangwei1; LIANG Zhuowen2; YANG Zhi3; 1. The Second Clinical Medical College of Shaanxi University of Chinese Medicine, Xianyang 712046, China 2. Department of Orthopedics, Xijing Hospital, Air Force Medical University, Xian 710032, China 3. Joint Revision Unit, Arthritis Hospital, Honghui Hospital, Xian 710054, China

Keywords:: Keywords: electromagnetic field osteoblast calcium ion cell proliferation cell differentiation

Abstract:: Abstract: Objective To explore the effects of electromagnetic field (EMF) on the change of Ca2+ in osteolbast from the qualitative and quantitative perspectives, and try to identify the role of Ca2+ in EMF regulation on osteoblast proliferation and differentiation. Methods A platform was established for generating sine EMF with a frequency of 38.7 Hz and a strength of 1.5 mT. The MC3T3-E1 osteoblasts were randomly divided into control group and experimental group (EMF intervention for 8 h per day). CCK8 was used to detect osteoblast proliferation, ALP staining to detect osteoblast differentiation, and Ca2+ fluorescence probes and flow cytometer to detect the Ca2+ concentration in osteoblasts. Results CCK8 result showed that EMF intervention for 48, 72, 96 and 120 h could significantly promote osteoblast proliferation. After 14 days of EMF intervention, the positive expression of ALP was significantly higher in EMF group than in control group. Ca2+ fluorescent staining and flow cytometry results revealed that EMF intervention could increase the Ca2+ in osteoblasts. Conclusion The EMF-induced upregulation of Ca2+ signal in osteoblasts may be closely related to the promotions of osteoblast proliferation and differentiation by EMF, but which Ca2+-related biosignaling pathways are involved in the EMF promoting osteoblast proliferation and differentiation remains to be further investigated.