Effects of pulsed low-dose rate radiotherapy on esophageal cancer cells and lung tissues(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2023年第5期
- Page:
- 536-539
- Research Field:
- 医学放射物理
- Publishing date:
Info
- Title:
- Effects of pulsed low-dose rate radiotherapy on esophageal cancer cells and lung tissues
- Author(s):
- WANG Liang1; JIN Fu2; XU Shan1; YANG Bo1; XIANG Shuang3; YANG Jiebin3; ZHANG Jun1; MOU Yanhong1; LUO Huanli2; LIU Qiang1
- 1. Cancer Center, Chongqing University Three Gorges Hospital, Chongqing 404100, China 2. Department of Radiation Oncology, Chongqing University Cancer Hospital, Chongqing 400030, China 3. Department of Pathology, Chongqing University Three Gorges Hospital, Chongqing 404100, China
- Keywords:
- Keywords: esophageal cancer pulsed low-dose rate radiotherapy transforming growth factor-β1 radiation-induced lung injury
- PACS:
- R815.2
- DOI:
- DOI:10.3969/j.issn.1005-202X.2023.05.002
- Abstract:
- Abstract: Objective To evaluate the responses of esophageal cancer cells and normal lung tissues in mice exposed to pulsed low-dose rate radiotherapy (PLDR). Methods Human esophageal cancer cell lines EC109 and KM mice (specific pathogen free) in 3 experiment groups were treated with conventional radiotherapy (CR, 5×2 Gy), hypo-fractionated radiotherapy (HR, 2×5 Gy), and PLDR ［5×(10×0.2) Gy, with a beam interval of 3 min］, respectively, and no radiation was delivered in control group (CG). CCK-8 was used to measure EC109 cell proliferation before irradiation and on days 1, 3, 5 and 7 after 10 Gy irradiation. At the 4th week after 10 Gy irradiation, the serum transforming growth factor-β1 (TGF-β1) level was detected using ELISA, and the lung tissue slices were stained with H&E to observe the morphologic changes. Results The relative optical densities and cell viabilities of EC109 cells in experimental groups were significantly lower than those in CG since the 3rd day after irradiation (P<0.05) and on days 5 and 7 after irradiation, the relative optical densities and cell viabilities of CR and PLDR groups were significantly higher than those in HR group (P<0.05) and there was no significant difference in relative optical densities and cell viabilities between CR and PLDR groups (P>0.05). At the 4th week after irradiation, CR and HR results in alveolar wall thickening and more inflammatory cells, but mice exposed to PLDR had normal lung tissues. CG and PLDR groups had similar serum TGF-β1 levels (P>0.05), which were lower than both CR and HR groups (P<0.05). Conclusion Compared with CR and HR, PLDR can significantly reduce radiation-induced lung injury while effectively inhibiting the proliferation of esophageal cancer cells.
Last Update: 2023-05-26