Quantitative analysis of the morphological structures of intranuclear inclusion bodies and nuclei in papillary thyroid carcinoma(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2023年第5期
- Page:
- 629-636
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Quantitative analysis of the morphological structures of intranuclear inclusion bodies and nuclei in papillary thyroid carcinoma
- Author(s):
- TAN Minhua1; CHEN Jiebin2; SHEN Dong3; XIA Yanhui1; ZHOU Yongjian1; AN Jianhong4; XIAN Xiaoyang1; SHEN Hong2
- 1. Department of Pathology, Zhaoqing First Peoples Hospital, Zhaoqing 526060, China 2. Department of Pathology, School of Basic Medicine (Department of Pathology, Nanfang Hospital), Southern Medical University, Guangzhou 510515, China 3. School of Public Health, Southern Medical University, Guangzhou 510515, China 4. School of Medicine, South China University of Technology, Guangzhou 510006, China
- Keywords:
- papillary thyroid cancer nucleus inclusion body morphometry quantitative pathology
- PACS:
- R365
- DOI:
- DOI:10.3969/j.issn.1005-202X.2023.05.017
- Abstract:
- Objective To quantify the geometrical and structural features of intranuclear inclusion bodies and nuclei in papillary thyroid carcinoma, and to provide quantitative diagnostic parameters for the pathological diagnosis of papillary thyroid carcinoma. Methods A total of 100 cases of thyroid papillary carcinoma were sectioned and stained with hematoxylin-eosin (HE). The images of inclusion bodies and nuclei were collected using a 40× objective lens, and their geometrical and structural features, including area, perimeter, long axis, short axis, Form PE, Form AR, common form factor (RFF), axial ratio, the area density of inclusion bodies and area ratio of inclusion bodies to the nucleoplasm, were obtained using Image-Pro Plus. Results (1) The area of intranuclear inclusion bodies in papillary thyroid carcinoma was approximately (13.575±9.045) μm2 (95% CI 13.339-13.810), and their area density in the nuclei was approximately 25.761%±10.683% (95% CI 25.483-26.039). The perimeter of the inclusion bodies was approximately (12.720±4.275) μm (95% CI 12.609-12.831 μm), with a long axis of (4.547±1.466) μm (95% CI 4.509~4.585) and a short axis of (3.482±1.178) μm (95% CI 3.451-3.512 μm). The axis ratio was approximately 0.772±0.121 (95% CI 0.769-0.775) Form PE, Form AR and RFF were approximately equal to 1 and the area ratio of inclusion bodies to the nucleoplasm was approximately 0.380±0.237 (95% CI 0.374-0.386). The coefficients of variations were 66.6%, 33.6%, 32.2% and 33.8% for the area, perimeter, long axis and short axis, respectively. (2) The area, perimeter, long and short axes, and their corresponding coefficients of variations were significantly greater in cancer cells with intranuclear inclusion bodies than in nuclei without intranuclear inclusion bodies (P<0.05). (3) The frequency distributions of the area, perimeter, long and short axes of intranuclear inclusion bodies, nuclei containing intranuclear inclusion bodies and nuclei without intranuclear inclusion bodies were all positively skewed. Conclusion (1) The intranuclear inclusion bodies in papillary thyroid carcinoma is roughly circular, with approximately 1/4 of the nucleus area and 1/2 of the nucleus perimeter, long axis, and short axis. These parameters all exhibited a large degree of variation. (2) The heterogeneity of the nucleus of carcinoma cells with inclusion bodies is apparent, and should be pay attention to during diagnosis. (3) The frequency distribution of the above mensioned parameters of inclusion bodies and nuclei are positively skewed.
Last Update: 2023-05-26