Experimental study of chronic lower limb pain rats with lumbar disc herniation based on resting-state functional magnetic resonance morphology(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2022年第4期
- Page:
- 430-435
- Research Field:
- 医学影像物理
- Publishing date:
Info
- Title:
- Experimental study of chronic lower limb pain rats with lumbar disc herniation based on resting-state functional magnetic resonance morphology
- Author(s):
- CHEN Qiqing1; ZHAO Jirong1; CAI Yi2; CHEN Wen1; ZHAO Ning1; MA Tong1; XUE Xu1; GUO Peiyao1; ZHANG Tianlong2; YANG Tao2; LI Weinong2; ZHANG Licun2
- 1. Second Department of Spine, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China 2. Clinical College of Traditional Chinese Medicine, Gansu University of Chinese Medicine, Lanzhou 730030, China
- Keywords:
- Keywords: lumbar disc herniation resting-state functional magnetic resonance imaging fractional amplitude of low frequency ?ctuation regional homogeneity
- PACS:
- R318；R681.5
- DOI:
- DOI:10.3969/j.issn.1005-202X.2022.04.007
- Abstract:
- Abstract: Objective To reveal the brain mechanism of chronic lower limb pain due to lumbar disc herniation (LDH) by observing the changes of brain structures and functions in rat model of LDH chronic lower limb pain using resting-state functional magnetic resonance imaging (RS-fMRI) and pain behavior assessment. Methods A total of 24 healthy SD male rats aged 6-8 weeks with a body weight of (250±20) g were randomly divided into 3 groups, namely normal group, sham operation group and model group. The LDH chronic lower limb pain rat model in model group was established by autologous nucleus pulposus transplantation. Gross behavioral observation, paw withdrawal threshold (PWT) and thermal withdrawal latency (TWL) measurements were carried out in all groups before modeling and on the 2nd, 7th, 14th, 21st, and 28th days after modeling. Before modeling and on the 14th and 28th day after modeling, 4 rats were randomly selected from each group to collect BOLD data using Bruker 7.0T nuclear magnetic field. The obtained BOLD data were processed by regional homogeneity (ReHo) and fractional amplitude of low-frequency fluctuation (fALFF) for statistical analysis. Results (1) There was no statistically significant difference in PWT and TWL among 3 groups of rats before modeling (P>0.05). At each time point after modeling, the differences in PWT and TWL between sham operation group and normal group were trivial (P>0.05), but the PWT and TWL in model group were different from those of normal group and sham operation group, with statistical significance (P<0.05). (2) The 3 groups of rats had an interactive effect on the brain structure at different time points, and the difference was statistically significant (P<0.05). The brain map results show that the ReHo value changed obviously in left periventricular grey matter and right striatum, and that the brain regions with significant differences in fALFF value included right striatum, left olfactory bulb and molecular layer of the cerebellum. Conclusion (1) After rats were modeled with LDH by autologous nucleus pulposus transplantation, PWT and TWL were significantly reduced, and hyperalgesia occurred, suggesting that LDH chronic lower limb pain rat model is successfully established. (2) The functional activities of some brain regions in LDH chronic lower limb pain rat model at resting state change significantly, which may be related to the occurrence and maintenance of chronic pain.
Last Update: 2022-04-27