Low-intensity pulsed ultrasound inhibits the proliferation of osteosarcoma cells through PI3K/AKT/mTOR signaling pathway(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第3期
- Page:
- 355-359
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Low-intensity pulsed ultrasound inhibits the proliferation of osteosarcoma cells through PI3K/AKT/mTOR signaling pathway
- Author(s):
- ZHANG Yu; MA Chenghua; JING Pengju; LI Peiwu
- Emergency Center, Lanzhou University Second Hospital, Lanzhou 730000, China
- Keywords:
- Keywords: low-intensity pulsed ultrasound phosphatidylinositol 3-kinase osteosarcoma proliferation signaling pathway
- PACS:
- R363；R738.1
- DOI:
- DOI:10.3969/j.issn.1005-202X.2021.03.017
- Abstract:
- Abstract: Objective To clarify the role and mechanism of low-intensity pulsed ultrasound (LIPUS) in regulating the proliferation of osteosarcoma cells, and to explore the potential value of LIPUS in the treatment of osteosarcoma. Methods In time gradient experiment, MG-63 cells were divided into LIPUS group and control group. MG-63 cells in LIPUS group were loaded with LIPUS for 1, 6, 12, 18 and 24 h. CCK-8 was used for detecting the cell proliferation in each group, and Western Blots for detecting the effects of 24 h LIPUS on PI3K/AKT/mTOR signaling pathway. In mechanism experiment, MG-63 cells were divided into control group, LY294002 group, LIPUS group and LIPUS+740Y-P group. After treating MG-63 cells with corresponding interventions in different groups, CCK-8 was used for detecting cell proliferation in each group, and Western Blots for detecting the expressions of proliferation-related proteins PCNA and Cyclin D1. After all data were repeated 3 times, one-way ANOVA was adopted for statistical analysis. Results After loading LIPUS for 18 and 24 h, the cell proliferation in LIPUS group was significantly lower than that in control group (P<0.010, P<0.001), which indicated that LIPUS could inhibit the proliferation of MG-63 cells. In addition, LIPUS could inhibit the expressions of p-PI3K, p-AKT and p-mTOR (P<0.001) (inhibiting the activation of PI3K/AKT/mTOR signaling pathway), without affecting the expressions of PI3K, AKT and mTOR. Like the inhibitor of PI3K (LY294002), LIPUS could inhibit the proliferation of MG-63 cells (P<0.001). The inhibition of LIPUS on proliferation of MG-63 cells could be reversed by 740Y-P (the agonist of PI3K) (P<0.001). These results revealed that LIPUS could inhibit the proliferation of osteosarcoma cells by inhibiting the activation of PI3K/AKT/mTOR signaling pathway. Conclusion LIPUS can inhibit the proliferation of osteosarcoma cells through PI3K/AKT/mTOR signaling pathway. LIPUS combined with chemotherapy or not may be one of the effective methods for the treatment of osteosarcoma.
Last Update: 2021-03-30