Chronic lower extremity pain due to lumbar disc herniation: an experimental study in rats using voxel-based magnetic resonance morphometry(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第7期
- Page:
- 820-825
- Research Field:
- 医学影像物理
- Publishing date:
Info
- Title:
- Chronic lower extremity pain due to lumbar disc herniation: an experimental study in rats using voxel-based magnetic resonance morphometry
- Author(s):
- ZHAO Jirong1; XUE Xu1; YUAN Yuzhe2; CHEN Qiqing1; CHEN Wen1; ZHANG Haiqing1; ZHAO Ning1; MA Tong1; ZHANG Tianlong3; YANG Tao3
- 1. Department of Spine Orthopedics, Gansu Provincial Hospital of TCM, Lanzhou 730050, China 2. School of Pharmacy, China Pharmaceutical University, Nanjing 210000, China 3. Graduate School, Gansu University of Chinese Medicine, Lanzhou 730030, China
- Keywords:
- Keywords: lumbar disc herniation magnetic resonance morphometry mechanical withdrawal threshold thermal withdrawal latency pain
- PACS:
- R318；R683.2
- DOI:
- DOI:10.3969/j.issn.1005-202X.2021.07.006
- Abstract:
- Abstract: Objective To study the correlation and characteristics of brain structural and morphological changes in rat models of chronic lower limb pain due to lumbar disc herniation (LDH) using voxel-based magnetic resonance morphometry (VBM) and pain behavioral observation, thereby revealing the brain mechanism of LDH chronic lower limb pain. Methods Twenty-four SD male rats aging 6-8 weeks old, with a body weight of (250±20) g, were selected as the research objects and randomly divided into 3 groups, namely Normal group, Sham-LDH group and LDH group and the rat model of LDH chronic lower limb pain was established by the transplantation of autologous nucleus pulposus. The pain behavioral observation was performed in each group before modeling and on the 2nd, 7th, 14th, 21st and 28th days after modeling, mainly including the measurements of mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL). Four rats in each group were randomly selected to collect T2-weighted structural images and VBM images before modeling and on the 14th and 28th days after modeling. Repeated measures analysis of variance was used for the statistical analysis of gray matter volume. Results There was no significant difference in MWT among 3 groups before modeling, without statistical differences (P>0.05). The MWT in Sham-LDH group and Normal group was similar on the 2nd, 7th, 14th, 21st and 28th days after modeling (P>0.05). In LDH group, MWT decreased significantly from the 2nd day to the 14th day after modeling, and then remained stable from the 14th day to the 28th day after modeling. On the 2nd, 7th, 14th, 21st and 28th days after modeling, the MWT in LDH group was statistically different from that in Normal group and Sham-LDH group (P<0.05). The comparison results of TWL were similar to those of MWT. There were interactions between the rat brain structures of 3 groups at different time points, and the differences were statistically significant (P<0.05). Gray matter volume was reduced in some brain regions, specifically, left hypothalamic region, left hippocampus subiculum, right secondary motor cortex, right striatum, right septal region, right cornu ammonis, corpus callosum and associated subcortical gray matter, molecular layer of the lateral cerebellum. Conclusion A rat model of LDH chronic lower limb pain is successfully established after the transplantation of autologous nucleus pulposus. Long-term reductions of MWT and TWL emerge and the hyperalgesia occurs after the modeling. The morphological changes in the brain structures of rat models of LDH chronic lower limb pain can be found using VBM.
Last Update: 2021-07-26