Effects of diffuse oxygen enrichment on the behavior and hippocampal CA1 area of mice with
traumatic brain injury at high altitude(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2020年第9期
- Page:
- 1198-1202
- Research Field:
- 医学人工智能
- Publishing date:
Info
- Title:
- Effects of diffuse oxygen enrichment on the behavior and hippocampal CA1 area of mice with
traumatic brain injury at high altitude
- Author(s):
- LI Yuanzhe1; YAN Xi2; RUAN Junyong3; ZHAI Mingming1; TANG Chi1; LUO Erping1; LIU Juan1
- 1. Department of Military Biomedical Engineering, Air Force Medical University, Xian 710032, China 2. Department of Dermatology,
the Second Affiliated Hospital of Air Force Medical University, Xian 710032, China 3. Department of Medical Engineering, Qingdao
Special Service Sanatorium Center of PLA Navy, Qingdao 266071, China
- Keywords:
- C57 mouse diffuse oxygen enrichment hypobaric hypoxia traumatic brain injury hippocampus
- PACS:
- R318；R338.2
- DOI:
- 10.3969/j.issn.1005-202X.2020.09.022
- Abstract:
- Objective To explore the effects of diffuse oxygen enrichment on the ethology and the hippocampus CA1 area of
mice with traumatic brain injury (TBI) in hypobaric hypoxia. Methods The hypobaric hypoxic chamber was used to simulate
the special environment of high altitude at 6 000 m, while the portable membrane oxygen enricher and IVC cage were used to
construct a diffuse oxygen-enriched cage. Twenty-one male C57 mice were randomly divided into TBI plain group (Sham
group), TBI hypoxia group (TLO group) and TBI oxygen enrichment group (THO group). The mice in TLO group and THO
group were placed in the simulated chamber for 1 week (24 h/d) and those in THO group were enriched with oxygen for 24 h
every day (30%±3%, V/V). The mice in Sham group were raised at the local altitude (about 400 m). After a week of
experiment, the body weight and behavioral indexes of the mice were measured, and the structure of the hippocampus was
also observed. Results There was no significant difference in body weight among 3 groups before the experiment (P>0.05).
After the experiment, the mice in both TLO group and THO group had significantly lower body weight than those in Sham
group (P<0.05), and the body weight of the mice in THO group was significantly higher than that of the mice in TLO group
(P<0.05). Moreover, the vertical activity of the mice in TLO group was significantly inferior to that of the mice in Sham
group (P<0.05) and compared with those of the mice in TLO group, the horizontal activity and vertical activity of the mice in
THO group were significantly increased (P<0.05). In TLO group, the nuclei of hippocampal pyramidal cells were shrinked in
volume, and the staining was deepened. However, the structural damage of hippocampal pyramidal cells in THO group was
significantly alleviated, and the arrangement was relatively neat. Conclusion The diffuse oxygen enrichment can effectively
prevent TBI mice at high altitude from hippocampal damages, reduce their irritability and anxiety and improve their
capability for space exploration.
Last Update: 2020-09-25