Abstract

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Title:: Preliminary experimental study of FAIMS in the detection of explosive wound samples

Author(s):: HE Qinghua; QI Haifeng; ZHONG Xiaozheng; ZENG Lin; LU Yanyi; LI Min; GUO Qingshan; State Key Laboratory of Trauma, Burns and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, China

Keywords:: explosive wound high-field asymmetric waveform ion mobility spectrometry bacterial detection

Abstract:: Objective To explore the feasibility of high-field asymmetric waveform ion mobility spectrometry (FAIMS) for the detection of wound samples. Methods Five wound samples (necrotic muscle, dead skin or pus) were collected from 3 patients with explosive wound. Two samples were got each time. One sample was sent to the laboratory for bacterial test, and the other was detected by FAIMS for the odor analysis of wound samples, thereby obtaining positive-mode and negative-mode FAIMS data. Results The laboratory results of the 5 samples showed that there was 1 case of Bacillus cereus, 1 case of Cupriavidus paucula, 1 case of Klebsiella pneumoniae and Enterobacter cloacae, and 2 cases of no bacterial growth. Some characteristic peaks were found in the positive-mode and negative-mode FAIMS for the detection of samples with bacterial infection. Moreover, the shapes and positions of the FAIMS characteristic peaks of the samples infected by different bacteria were different. No characteristic peaks appeared in the FAIMS of the bacteria-free samples. Conclusion FAIMS can reflect the infection of the wound samples to a certain extent, and the FAIMS profiles of the samples infected by different bacteria were different, suggesting that FAIMS can be used to distinguish the type of wound infection. FAIMS is a potential technique for the rapid detection of wound infections and is worthy of further experimental research.