Abstract

Info

Title:: Effects of pulsed electromagnetic field on hepatic oxidative stress of mice in simulated high-altitude environment

Author(s):: ZHAI Mingming; LIU Juan; LUO Erping; TANG Chi; School of Biomedical Engineering, Air Force Medical University, Xi’an 710032, China

Keywords:: liver; oxidative stress; pulsed electromagnetic field; simulated high-altitude environment

Abstract:: Objective To investigate the effects of pulsed electromagnetic field on the hepatic oxidative stress of mice in simulated high-altitude environment. Methods Fifteen male SPF mice were randomly divided into plain control group (Control group), hypobaric hypoxia group (HH group) and pulsed electromagnetic field intervention group (PEMF group). The mice in HH and PEMF groups were set in a low pressure and low oxygen cabin which was used to simulate an altitude of 6 000 m, so as to construct a mouse model of hepatic oxidative stress. The mice in PEMF group were treated with 2 mT magnetic field per day for 2 h. All mice were sacrificed at 5 days after the experiment, and samples were collected. The biomarkers of oxidative stress in hepatic tissues were detected, and the expression of antioxidant enzymes active protein was detected. Results Compared with those in HH group, the levels of methane dicarboxylic aldehyde and oxidized glutathione in PEMF group were reduced obviously, while the level of reduced glutathione was increased significantly, and the glutathione peroxidase activity in PEMF group was increased dramatically, which was consistent with protein expression activity. Conclusion Pulsed electromagnetic field can improve the imbalance of hepatic oxidative stress in mice which was caused by simulated high-altitude environment, and significantly increase the activity of glutathione peroxidase.