Abstract

Info

Title:: Development and application of a new pressure loading apparatus for cells in vitro

Author(s):: LIANG Qian1; YANG Zi’nan2; 1. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Diseases, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China; 2. Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China

Keywords:: Keywords: pressure loading apparatus; cell mechanics; hydrostatic pressure

Abstract:: Abstract: Objective To design a new pressure loading apparatus for cells in vitro for simulating the hydrostatic pressure on cells in vitro, thereby providing a foundation for the further study on the changes of cells in vitro under hydrostatic pressure. Methods The designed apparatus consisted of a pressure chamber, a micropressure sensor, a programmable controller and a mini air pump. The running status of the mini air pump was regulated by the control program coded on the programmable controller, so that the hydrostatic pressure on cells was controlled by regulating the gas pressure inside the chamber. Results The loading pressure of the apparatus ranged from 0 to 120 mmHg, with a fluctuation range of ±2 mmHg. The common cell culture flask or plate could be steadily put in the pressure chamber and loaded with preset hydrostatic pressure. Conclusion The designed pressure loading apparatus has high accuracy and reliability, and it is suitable for studying the effects of constant hydrostatic pressure on cells in vitro.