Effects of cofilin on the cytoskeleton and phenotype of chondrocytes(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2020年第1期
- Page:
- 107-114
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Effects of cofilin on the cytoskeleton and phenotype of chondrocytes
- Author(s):
- LIU Renfeng1; CHEN Cheng2; WU Jiangyi2; YANG Liu2; SONG Xiongbo2; HUANG Shu1; LIU Baorong1
- 1. Department of Orthopaedics, the First Affiliated Hospital of Hu’nan Normal University, Changsha 410005, China; 2. Department of Joint Surgery, Southwest Hospital, Army Medical University, Chongqing 400038, China
- Keywords:
- Keywords: cofilin; chondrocyte; cytoskeleton; transfection
- PACS:
- R318.04；R329
- DOI:
- DOI:10.3969/j.issn.1005-202X.2020.01.020
- Abstract:
- Abstract: Objective To study the effect of cofilin expression on the phenotype and skeleton of chondrocytes. Methods The low- and high-level cofilin expression models were constructed by siRNA and plasmid transfection. The cytoskeletons and related proteins in chondrocytes which had different level of cofilin expression were detected for studying the effects of cofilin on the phenotype and skeleton of chondrocytes. Results After reducing cofilin expression, the cytoskeleton of chondrocytes observed after phalloidin staining became more compact, and the actin density in the cell extension direction was denser than that of control group. Meanwhile the expression of COLⅠ in chondrocytes which was 137.42%[±]25.14% was slightly increased, without statistical significance (P>0.05), but the expression of COLⅡ which was 19.96%[±]14.59% was significantly decreased, with statistical significance (P<0.05). When increasing cofilin expression in chondrocytes, the immunofluorescence intensity of the cytoskeleton was slightly weaker than that of control group, and the distribution was slightly wider. Meanwhile the expression of COLⅠ which was 44.47%[±]21.67% was decreased, with statistical significance (P<0.05), and the expression of COLⅡ which was 116.16%[±]13.18% was slightly increased, without statistical significance (P>0.05). Conclusion Cofilin which helps chondrocytes maintain their characteristics plays an important role in the cytoskeleton of chondrocyte. The decreased expression of cofilin will lead to the change of chondrocyte characteristics, resulting in cartilage damage-related diseases. It could be a potential therapeutic method to treat cartilage damage by regulating the expression of cofilin, but further researches are required to clarify the relevant mechanisms.
Last Update: 2020-01-14