1H-proton magnetic resonance spectroscopy for studying effects of L-band microwave radiation on learning and memory functions of rats(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2019年第12期
- Page:
- 1373-1376
- Research Field:
- 医学放射物理
- Publishing date:
Info
- Title:
- 1H-proton magnetic resonance spectroscopy for studying effects of L-band microwave radiation on learning and memory functions of rats
- Author(s):
- WANG Haoyu1; LI Chunfang1; 2; DONG Ji1; ZHAO Li1; ZHANG Jing1; WANG Hui1; XU Xinping1; YAO Binwei1; ZHOU Hongmei1; LI Jiakai3; MA Lin2; PENG Ruiyun1
- 1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China; 2. Department of Radiodiagnosis, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China; 3. Department of Radiodiagnosis, Hainan Hospital of PLA General Hospital, Sanya 572013, China
- Keywords:
- Keywords: rat; microwave radiation; 1H-proton magnetic resonance spectroscopy; neuron; learning and memory
- PACS:
- R318；R811.1
- DOI:
- DOI:10.3969/j.issn.1005-202X.2019.12.002
- Abstract:
- Abstract: Objective To investigate the effects of L-band microwave radiation on the learning and memory functions of rats based on 1H-proton magnetic resonance spectroscopy (1H-MRS). Methods Twenty-six SPF male Wistar rats were randomly divided into Sham group and 30 mW/cm2 L-band microwave radiation group (L30 group). The rats in L30 group were restrained in plastic cages and exposure to 30 mW/cm2 L-band microwave radiation for 10 min. The incident direction of the microwave was from dorsal to ventral. The rats in Sham group were also restrained in the same cages for 10 min, without any microwave radiation. Both learning and memory functions of rats were evaluated at 1 d and 7 d after the radiation using Morris water maze. The level of N-acetylaspartate (NAA), choline (Cho) and creatine (Cr) in the rats’ brain were measured with 1H-MRS. Results Compared with that of Sham group, the average escaping latency of L30 group at 1 d and 7 d after microwave radiation was prolonged, but there was no statistical significance between two groups (P>0.05). The results of 1H-MRS examination demonstrated that at 1 d after the radiation, the NAA/Cr of L30 group was significantly lower than that of Sham group (P<0.05), while the Cho/Cr was similar in two groups, without significant differences (P>0.05); and that at 7 d after the radiation, there was no significant difference in both of NAA/Cr and Cho/Cr between L30 group and Sham group (P>0.05). Conclusion 30 mW/cm2 L-band microwave radiation can lead to a significant decrease of intracerebral NAA/Cr in rat at 1 d after microwave exposure, without resulting in obvious changes in the behavioral indexes of learning and memory, which suggests that 1H-MRS can be used for evaluating the microwave radiation induced damage to neurons related to learning and memory before any significant changes of behavioral measurements can be observed.
Last Update: 2019-12-24