Abstract

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Title:: Inhibitory effects of liposome curcumin combined with sorafenib on human hepatocellular carcinoma Huh7 cells

Author(s):: ZHU Yiqing; WANG Fang; Department of Medical Genetics, the Second Military Medical University, Shanghai 200433, China

Keywords:: ; Keywords: liver cancer; liposomes curcumin; sorafenib; combination therapy

Abstract:: Abstract: Objective To investigate the inhibition of the combination therapy of liposome curcumin and sorafenib on hepatocellular carcinoma Huh7 cells. Methods Liposome curcumin was prepared by liposome embedding technique, and its particle size and Zeta potential were determined by Nano-ZS laser particle size analyzer. Human hepatocellular carcinoma Huh7 cells were treated with liposome curcumin (10 ?mol/L) and sorafenib (0.1 ?mol/L) separately and jointly. The effects of liposome curcumin and sorafenib on the proliferation of Huh7 cells were evaluated with CCK8 assay and EdU assay, and their effects on the migration of Huh7 cells were detected by Transwell chamber migration assay. Results Liposome curcumin with a diameter of (118.7±13.6) nm and Zeta potential of (-9.8±1.1) mV was obtained. Using liposome curcumin alone significantly inhibited the proliferation and migration of Huh7 cells. The combined use of liposomal curcumin and sorafenib remarkably improved the inhibitory effects of sorafenib on the proliferation and migration of Huh7 cells. Conclusion The combined use of liposome curcumin and sorafenib which can inhibit the proliferation and migration of hepatoma Huh7 cells is a potential strategy for the treatment of hepatocellular carcinoma.