Microfluidic technology-based method for sorting circulating tumor cells(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2019年第2期
- Page:
- 229-233
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Microfluidic technology-based method for sorting circulating tumor cells
- Author(s):
- WANG Zhengyuan; XU Xiulin; WANG Yan
- School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
- Keywords:
- ; Keywords: circulating tumor cells; microfluidic technology; sorting; enrichment; cancer diagnosis; review
- PACS:
- R318
- DOI:
- DOI:10.3969/j.issn.1005-202X.2019.02.020
- Abstract:
- Abstract: Circulating tumor cells (CTCs) are mainly derived from the peripheral blood of tumor tissue that is spontaneously shedding. CTCs have an important influence on the spread and metastasis of malignant tumors and have gradually been considered as a marker of distant metastasis of tumors. The selection, enrichment and detection of a very small amount of specific and sensitive CTCs in peripheral blood are not only helpful for the early diagnosis of tumors, the evaluation of efficacy and the monitoring of recurrence and metastasis, but also conducive to the identification of CTCs and the genome and transcriptome sequencings of downstream single cells, providing a new strategy for targeted therapy of tumors, which is of great significance in personalized medicine. Microfluidics can integrate physical, chemical and biological methods at the microscale to achieve high-throughput, high-efficiency, low-cost sorting and enrichment of a small amount of CTCs. This technology has been rapidly developed in recent years and has been widely applied. Herein, the latest research progresses on microfluidic chip systems in the sorting, enrichment and capture of CTCs are summarized. Moreover, the principle and application examples of the unmarked or labeled sorting method are expounded, and the advantages and disadvantages of various technologies are analyzed. The problems existing at the stage are also discussed. Finally, the applications and future development trends of microfluidic CTCs sorting chips are prospected.
Last Update: 2019-02-26