Transcriptional analysis of gene signatures in normal lung tissues after an acute exposure to heavy-ion irradiation(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2019年第2期
- Page:
- 136-141
- Research Field:
- 医学放射物理
- Publishing date:
Info
- Title:
- Transcriptional analysis of gene signatures in normal lung tissues after an acute exposure to heavy-ion irradiation
- Author(s):
- GENG Jiwu1; ZHOU Zhaoming2; SHAN Changguo2; WEN Lei2; LIU Hao3; CHENG Jie4; ZHOU Meijuan3; CHEN Longhua5; CAI Linbo2; ZHOU Cheng2
- 1. Guangdong Key Laboratory of Occupational Disease Prevention and Treatment/Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China; 2. Department of Radiation Oncology, Guangdong Sanjiu Brain Hospital, Guangzhou 510510, China; 3. Department of Radiation Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China;4. Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China; 5. Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
- Keywords:
- ; Keywords: heavy charged particle radiotherapy; stress response; radiation-induced lung injury; genome-wide transcriptional analysis; biomarker
- PACS:
- R312；R146
- DOI:
- DOI:10.3969/j.issn.1005-202X.2019.02.003
- Abstract:
- Abstract: Objective To investigate the potential gene-signatures for normal tissue stress response after an acute exposure of heavy-ionirradiation in lung. Methods Female C57BL6 mice aging 8-10 weeks old were randomly assigned into irradiation group (12.5 Gy) and non-irradiation group (0 Gy). The mice in irradiation group were exposure to heavy-ion irradiation, while those in non-irradiation group were exposure to sham irradiation. Gene chip was used to carry out genome-wide transcriptional analysis on lung tissues which were extracted at 2 and 24 hours after irradiation. Moreover, a dose-escalated irradiation was performed on mice to analyze the dose-effect relationship of heavy ion-sensitive genes at the 7th day after irradiation. Results At 2 hours after irradiation, the mRNA expression levels of Trp53inp1, Phlda3, Ddit4l, Gtse1, Sesn2, Bbc3, Mdm2, Ptp4a1, Pmaip1, and Osgin1 were found to be substantially up-regulated in mice lung tissues, while the mRNA expression levels of Wisp2, IL33, Dido1, Efcab4a, and Myo1f were apparently down-regulated. At 24 hours after irradiation, the expression levels of 9 genes, namely Phlda3, Ddit4l, Trp53inp1, Gtse1, Sesn2, Exoc4, Ephx1, Thyn1 and Ei24 were increased remarkably, while the expression levels of Gpihbp1, Sla, and Hist1h3ah were obviously down-regulated. The most up-regulated gene-panel after acute exposure to heavy-ion irradiation was further validated by 7 days of dose-escalated irradiation. Conclusion Five genes, namely Trp53inp1, Phlda3, Sesn2, Gtse1 and Ddit4l, can be taken as the potential markers for detecting the stress response of lung tissues after high-linear energy transfer irradiation. Further investigations on PCR and protein levels are needed to substantiate these findings.
Last Update: 2019-02-26