Abstract

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Title:: Effects of loading time of fluid shear stress on expression of piezo1 mechanosensitive protein in osteoblasts

Author(s):: YAN Liang1; 2; 3; JIANG Jin1; 2; 3; ZHANG Xiaohui1; 2; 3; WAN Lang1; 2; 3; MA Chongwen1; 2; 3; LI Rui1; 2; 3; XIA Yayi1; 2; 3; 1. Lanzhou University, Lanzhou 730000, China; 2. Department of Orthopedics, Second Hospital of Lanzhou University, Lanzhou 730000, China; 2. Gansu Key Laboratory of Orthopaedics, Lanzhou 730000, China

Keywords:: ; Keywords: fluid shear stress; MC3T3- E1 cell; piezo1 protein; osteoblasts; mechanosensitive ion channel

Abstract:: Abstract: Objective To discuss the variations of the expression of piezo1 mechanosensitive ion protein in MC3T3-E1 osteoblasts with the increasing loading time of fluid shear stress (FSS). Methods With the use of a self-designed parallel plate flow system, FSS (12 dyn/cm2) was acted on MC3T3-E1 osteoblasts for 0, 15, 30, 45, 60, 90 min. The expression level of piezo1 mechanosensitive ion protein was detected by immunofluorescence. Results Piezo1 was obviously expressed in the cytoplasm and nucleus of osteoblasts, especially in the cytoplasm. FSS of 12 dyn/cm 2 was acted on MC3T3-E1 cells cultured in vitro. With the increase of loading time, the expression of piezo1 protein was upregulated. At 45 minutes after loading, the expression of piezo1 protein reached the maximum. Conclusion Loading 12 dyn/cm2 FSS can upgrade the expression of piezo1 mechanosensitive ion protein in MC3T3-E1 osteoblasts, and the optimal loading time of FSS is 45 minutes.