Abstract

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Title:: Preparation of bone tissue engineering scaffold with aspartic acid-modified nano-hydroxyapatite/poly L-lactic acid and its bio-characterizations

Author(s):: HUANG Shu; WU Jiangyi; YANG Junjun; ZHOU Yizhao; LIU Baorong; XIA Duo; LIU Renfeng; Department of Orthopedics, Hunan Provincial People’s Hospital (the First Affiliated Hospital of Hunan Normal University), Changsha 410005, China

Keywords:: ; Keywords: nano-hydroxyapatite; poly L-lactic acid; aspartic acid; composite; biocompatibility

Abstract:: Abstract: Objective To design and prepare surface modified cartilage tissue scaffolds with the use of aspartic acid-modified nano-hydroxyapatite (Asp-nHA) and poly L-lactic acid (PLLA), and evaluate its feasibility as a scaffold for cartilage tissue engineering through in vitro experiment. Methods Asp-nHA was synthesized with the standard EDC/NHS coupling method, and the resultant Asp-nHA was conjugated to PLLA to form Asp-nHA/PLLA composite scaffold. The osteoblast adhesion, expansion and proliferation on Asp-nHA/PLLA composite scaffold were evaluated. Results Osteoblast adhesion, expansion and proliferation on Asp-nHA/PLLA composite scaffold were significantly increased due to the addition of nHA. The in vitro analysis illustrated that Asp-nHA/PLLA composite was superior to PLLA and HA/PLLA in bio-characterizations and had the strongest ability to induce osteogenic differentiation in vitro. Conclusion Asp-nHA/PLLA is simple to be prepared and its good biocompatibility and osteogenic activity make it become a good kind of scaffold for cartilage tissue engineering.