Abstract

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Title:: Structural design and functional analysis of an apparatus for detection of circulating tumor cells

Author(s):: GUO Bingbing; HU Xiufang; SONG Ziyue; HUANG Xinyu; SHEN Yichun; ZOU Renling; GU Xuelian; DI Yuanshuai; GUO Wanxing; XU Xiulin; School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

Keywords:: Keywords: microfluidic high throughput circulating tumor cells early screening

Abstract:: Abstract: In order to meet the needs of patients for high-quality and precision diagnosis and treatment services, and to achieve intelligent and high-throughput non-invasive early screening with high detection rate, an apparatus for the detection of circulating tumor cells (CTCs) is developed based on microfluidic technology. The apparatus provides highly-integrated and automated microfluidic chips, and uses precise and efficient fluorescence detection system for CTCs detection at the single cell level. The programmed detection is realized through the detection system. The apparatus can be used to separate, enrich and detect CTCs in patients blood. CTCs enrichment liquid is obtained from human peripheral blood samples by microfluidic sorting chip, and then the number of CTCs in blood samples is counted by upper computer through microfluidic detection chip and optical path detection system. The experiment on the simulated blood samples after red blood cell lysis is carried out for 6 times. At the optimal flow velocity of 0.2 mL/h, the CTCs detection rate is higher than 75.9% on average, and the intraclass correlation coefficient is greater than 0.8. It is proved that the apparatus has high detection rate and good reliability, and it can serve as a high-quality research facility for promoting technical breakthroughs in related fields.