Effects of diabetes on bone biomechanics in ovariectomized rats(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2018年第9期
- Page:
- 1099-1103
- Research Field:
- 生物材料与力学
- Publishing date:
Info
- Title:
- Effects of diabetes on bone biomechanics in ovariectomized rats
- Author(s):
- XIE Qiqi1; 2; 3; LIWenzhou1; 2; 3; DENG Yajun1; 2; 3; SHIWeidong1; 2; 3; REN Enhui1; 2; 3; MAJinglin2; PAN Yunyan1; KANG Xuewen1; 3; WANG Jing1; 2; 3
- 1. Department of Orthopaedics, Lanzhou University Second Hospital, Lanzhou 730000, China; 2. Key Laboratory of Osteoarticular
Disease of Gansu Province, Lanzhou 730000, China; 3. Lanzhou University, Lanzhou 730000, China
- Keywords:
- Keywords: type I diabetes; ovariectomized rat; osteoporosis; bone mineral density; biomechanics
- PACS:
- R318.01
- DOI:
- 10.3969/j.issn.1005-202X.2018.09.019
- Abstract:
- Abstract: Objective To investigate the effects of type I diabetes on bone mineral density (BMD) and biomechanical parameters
in ovariectomized rats. Methods Forty female Sprague-Dawley rats aged 3 months of SPF were randomly divided into 4 groups,
namely sham operation group (Sham group), simple ovariectomized group (group A), diabetes group (group B), and diabetes
combined with ovariectomized group (group C), with 10 rats in each group. The rats in group A were subjected to bilateral
oophorectomy to establish osteoporotic rat models, and the rats in group B were treated with intraperitoneal injection of
streptozotocin (STZ) to establish diabetic rat models. After 7 days of surgical removal of the ovary, the rats in group C were
intraperitoneally injected with STZ and underwent lumbar compression test and femoral three-point bend test to prepare diabetes
combined with ovariectomized models. The rats were kept for 3 months after modeling. Then the rats were sacrificed to collect
lumbar vertebrae and femoral bone for dual-energy X-ray examination and biomechanical examination. Results The BMD in
group A, B and C was significantly lower than that in Sham group, with statistical significance (P<0.05), and the most significant
BMD decrease in regions of interest was observed in group C, followed by group B and then group A. In group A, the BMD
decreases at lumbar spine (the whole segment, L6, L4, L3), the whole femur and its distal and proximal ends were the most obvious.
In group B, the BMD decreases were mainly found at the lumbar vertebra (the whole segment, L3, L2, L1) femurs, and the distal
and proximal ends of the femur. In group C, the BMD at all regions of interest were significantly decreased, and the decreases at the lumbar vertebrae (the whole segment, L1, L2, L3, L4), and the distal and proximal ends of the femur were relatively obvious
when compared with the BMD decrease at the L6. Compared with Sham group, group A, B and C showed significant decreases
in the biomechanical parameters obtained with femoral three-point bending test and lumbar compression test, with a downtrend
consistent with the change trend of BMD, and the decline in Group C was the most significant. Conclusion Type I diabetes
aggravates the decrease of BMD and biomechanics in ovariectomized rats, but the site of bone loss sensitivity is not the same
as that of ovariectomized rats. For ovariectomized rats, the most sensitive areas of bone loss were the lumbar vertebrae (the whole
segment, L6), and the distal and proximal ends of the femur; for diabetes rats, those were lumbar vertebrae (the whole segment,
L3, L2, L1), and the distal and proximal ends of the femur; and for diabetes combined with ovariectomized rats, those were lumbar
vertebrae (L1, L2, L3, L4, the whole segments), distal and proximal ends of the femur.
Last Update: 2018-09-28