Expression of ARHGAP10 in lung adenocarcinoma and relevant mechanisms(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2023年第5期
- Page:
- 653-660
- Research Field:
- 其他（激光医学等）
- Publishing date:
Info
- Title:
- Expression of ARHGAP10 in lung adenocarcinoma and relevant mechanisms
- Author(s):
- LIU Liwei1; 2; LIN Junhao1; CHEN Fengsheng1
- 1. Cancer Center, Hospital of Integrated TCM and Western Medicine, Southern Medical University, Guangzhou 510315, China 2. Affiliated Hexian Memorial Hospital, Southern Medical University (Hexian Memorial Hospital of Panyu District), Guangzhou 511400, China
- Keywords:
- Keywords: Rho GTPase activating protein 10 lung adenocarcinoma immunotherapy bioinformatics analysis
- PACS:
- -
- DOI:
- 1005-202X（2023）05-0653-08
- Abstract:
- Abstract: Objective To investigate the role and potential molecular mechanisms of Rho GTPase activating protein 10 (ARHGAP10) in the occurrence and development of lung adenocarcinoma using bioinformatics analysis, thereby providing a bioinformatics basis for further study. Methods The high-throughput RNA sequences of lung adenocarcinoma patients and relative clinical data were obtained from TCGA database which included cancer tissue from 514 lung adenocarcinoma patients and adjacent normal tissues from 59 patients. GSE115002 lung adenocarcinoma gene chip data was downloaded from GEO, and the chip contained the cancer tissue and adjacent normal tissues from 52 lung adenocarcinoma patients and the matched normal adjacent tissues. The differences in the expression of ARHGAP10 between lung adenocarcinoma tissues and adjacent normal tissues in TCGA and GSE115002 were analyzed using R language, and the correlation between ARHGAP10 expression and clinical prognosis of lung cancer patients was further explored. GO and KEGG pathway enrichment analyses were carried out on the overlapping genes in the two databases. The STRING database was used to construct the interaction protein network of ARHGAP10-related genes (ARGs), and the correlation between ARHGAP10 and immune infiltration was analyzed using TIMER database. Results ARHGAP10 had lower expression in lung adenocarcinoma tissues than in adjacent normal tissues (P<0.01), and lung adenocarcinoma patients with high ARHGAP10 expression had longer overall survival and progression-free survival (P<0.05). There were 133 ARGs in the TCGA and GSE115002 databases, involving MAPK, PI3K-AKT signaling pathway, extracellular matrix receptor binding, actin cytoskeleton, tumor signaling pathway and other biological processes. PRELP, LIMS2, PPAP2B, MRPL42, SRP9 and TSNAX were found to play key roles in the protein interaction network of ARGs. ARHGAP10 was positively correlated with DC cells and neutrophil infiltration, as well as PD-L1 expression. Conclusion ARHGAP10 which has an inhibitory effect on lung adenocarcinoma and is positively correlated with the survival and clinical prognosis of lung adenocarcinoma can serve as a potential prognostic marker for lung adenocarcinoma.
Last Update: 2023-05-26