Abstract

Info

Title:: Dynamic assessment of inflammation in mice with cerebral ischemia-reperfusion injury using gadoxetate disodium-enhanced magnetic resonance imaging

Author(s):: ZHAO Yiling; RUAN Peng; HE Li; LUO Shiyong; ZHOU Lang; YANG Fan; Department of Radiology, Wuhan Third Hospital, Wuhan 430074, China

Keywords:: Keywords: cerebral ischemia-reperfusion disodium gadoxetate inflammatory response magnetic resonance

Abstract:: Abstract: Objective To investigate the dynamic changes of inflammatory responses in mice with cerebral ischemia-reperfusion injury using gadoxetate disodium-enhanced magnetic resonance imaging. Methods A C57BL/6n mouse model of cerebral ischemia-reperfusion was established by blocking the right middle cerebral artery with the closed suture. Subsequently, gadoxetate disodium was injected into the tail vein of the mice, and MRI scans were performed at the corresponding time points to observe the signal changes. In addition, immunohistochemical techniques were used to measure the levels of inflammatory cytokines in brain tissues. Results There were no significant differences in the levels of tumor necrosis factor-α and interleukin-1β between the middle cerebral artery occlusion (MCAO) group and MCAO + gadoxetate disodium group. The lowest signal ratio in the negative enhancement region was reduced after reperfusion in mice injected with disodium gadolinium. Conclusion Gadoxetate disodium injection does not affect the inflammatory response during acute cerebral ischemia-reperfusion. Therefore, gadoxetate disodium-enhanced magnetic resonance imaging can be used as an effective means to monitor the inflammatory response during acute cerebral ischemia-reperfusion.