Abstract

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Title:: Effects of different spectral LEDs on endoplasmic reticulum stress in cone cells

Author(s):: CHEN Wenyi1; LIN Rong1; XIAO Kunhong1; YUAN Kun2; CHEN Zhongjiang1; HUANG Yan1; 1. Department of Ophthalmic Optics, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China 2. Division of Prevention and Community Health, Fuwai Hospital and National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100000, China

Keywords:: light emitting diode sun-like spectrum cone cell endoplasmic reticulum stress

Abstract:: Abstract: Objective To investigate the endoplasmic reticulum (ER) stress in mouse cone cells (661W cells) exposed to sun-like spectral LED and conventional white LED. Methods 661W cells were divided into normal control group (NC) for cell culture in darkness, SL groups for 3 000 lux sun-like spectral LED exposure for 6-24 h, and CL groups for 3 000 lux conventional white LED exposure for 6-24 h. Cell state was observed using optical microscopy, and CCK-8 assay was used to detect cell viability. The ER stress-related genes and protein expression levels were detected with real-time fluorescence quantification PCR (RT-qPCR) and Western blot. Results Compared with that in NC group, the rate of cell death in LED exposure groups was increased with time, and the cell viability was decreased significantly (P<0.05), but the cell damages were lighter in SL groups than in CL groups. CL exposure increased the expression levels of ATF4 mRNA, CHOP mRNA and ATF4 protein (P<0.01). The expression levels of GRP78/Bip mRNA and protein were significantly increased in CL-6 h group (P<0.01), and then decreased significantly. SL-24 h group improved the expression levels of ATF4 protein (P<0.05). Conclusion Conventional white LED can result in ER stress and induce cone cell damage, but the responses caused by sun-like spectral LED is significantly reduced.