Quantitative pathological test and analysis of structural changes of islets in streptozotocin-induced diabetic mice(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2023年第5期
- Page:
- 622-628
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Quantitative pathological test and analysis of structural changes of islets in streptozotocin-induced diabetic mice
- Author(s):
- CHEN Chunya1; LI Chunyan1; AN Jianhong1; SHEN Hong1; 2
- 1. School of Medicine, South China University of Technology, Guangzhou 510006, China 2. Department of Pathology, School of Basic Medicine (Department of Pathology, Nanfang Hospital), Southern Medical University, Guangzhou 510515, China
- Keywords:
- Keywords: diabetes islet quantitative pathology positive unit streptozotocin
- PACS:
- R365
- DOI:
- DOI:10.3969/j.issn.1005-202X.2023.05.016
- Abstract:
- Abstract: Objective To quantitatively reveal the structural changes of pancreatic islets in mice with streptozotocin-induced diabetes, and to provide a pathological basis for the analysis of changes in islet function. Methods Intraperitoneal injection of streptozotocin (STZ) induced diabetes in Balb/c mice. After the mice were sacrificed by excessive CO2, the pancreas tissues were taken for routine pathological sample preparation, insulin immunohistochemical staining, image test and quantitative analysis. Results At the 8th week of modeling, the blood glucose of diabetic group (DM group) was significantly higher than that of normal group (NC group), and the mean blood glucose of DM group was 19.3 mmol/L. Compared with NC group, DM group had less islets, fewer β cells and reduced β cell area (P<0.05), and it was calculated that β cell count, β cell area, and the expression of positive unit of insulin were decreased by 62.7%, 27.0%, and 53.8%, respectively. The area density (AAβ, PI), numerical density (NAβ, PI) and number percentage (βPer) of pancreatic β cells in DM group were significantly decreased (P<0.05). PU, AAβ, PI and βPer were negatively correlated with the rise of blood glucose, with Pearson correlation coefficients (R value) of -0.653, -0.736 and -0.899, respectively (P<0.05). Moreover, a function of modeling time (t) and the percentage change of pancreatic β cell count was established: [βPer(%)=79.68-9.74t+0.38t2+0.06t3-4.66×10-3t4+2.86×10-5t5+1.68×10-5t6]. Conclusion Measuring the indicators related to the morphological changes of β cells and the pancreatic islets and in diabetic mice models using quantitative pathological technique can help accurately evaluate the damage of pancreatic islets, and establish the mathematical model of blood glucose and the measured values for predicting the damage of pancreatic islets caused by the rise of blood glucose.
Last Update: 2023-05-26