Abstract

Info

Title:: Impedance characteristics in the interaction process between blood and concanavalin agglutinin

Author(s):: LING Li; ZHONG Xianhua; LI Zhangyong; School of Bioinformatics, Chongqing University of Posts and Telecommunications, Chongqing 400065, China

Keywords:: Keywords: hemagglutination concanavalin agglutinin impedance characteristic characteristic frequency

Abstract:: Abstract: Objective To study the impedance characteristics of blood cells in the process of blood coagulation formation by the interaction of blood and concanavalin agglutinin. Methods Rabbit blood cells and concanavalin agglutinin were selected to form the experimental group. An impedance monitoring platform which was built based on precise impedance analyzer was used to continuously collect the impedance characteristic parameters such as complex impedance amplitude modulus, phase angle, frequency, real value, imaginary value during the reaction process in each experimental group for further collation and analysis. Results After adding 10, 20 and 30 mg/mL of concanavalin agglutinin into a 50% hematocrit of rabbit blood cell suspension, the characteristic frequency of rabbit blood cells was increased from 19.80 MHz to (27.31±2.19) MHz. At the characteristic frequency, with the increase of the reaction time, the average increment of the complex impedance amplitude modulus was (-18.27±1.29) Ω, and the average increment of the phase angle was 3.86°±0.38°. However, the blank control group without adding concanavalin agglutinin showed the opposite trends. Conclusion Concanavalin agglutinin can increase the characteristic frequency of rabbit blood cells, decrease complex impedance amplitude modulus, enlarge phase angle, and speed up the agglutination.