Abstract

Info

Title:: Real-time monitoring of heparin concentration using microdialysis-microfluidics system

Author(s):: ZHOU Xingbei1; 2; DING Tao1; WANG Shushui1; 2; 1. School of Medicine, South China University of Technology, Guangzhou 510006, China 2. Department of Pediatric Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial Peoples Hospital (Guangdong Academy of Medical Sciences), Guangzhou 510080, China

Keywords:: Keywords: heparin microdialysis microfluidics real-time monitoring biochemical detection

Abstract:: Abstract: Objective To provide a novel method for detecting the heparin concentration in blood rapidly, continuously and accurately, and guiding the administration of protamine. Methods Both microdialysis technology and microfluidics technology were optimized and then integrated into a microdialysis-microfluidics system for the real-time monitoring of heparin concentration in human plasma. Results The microdialysis-microfluidics system could quantitatively measure the heparin within the concentration range of 0-4.0 IU/mL, with the function of automatic reading every 30 s. The duration from sampling to result output was 16 min. Moreover, the results output by the system had a high consistency with those obtained by the conventional microplate reader (R=0.995). Conclusion The proposed method can not only monitor the heparin concentration which was within therapeutic range rapidly and accurately, but also assess the dose of protamine, thereby guiding drug administration.