Comparison of temperature open-loop and closed-loop feedbacks in high-voltage pulsed electric field ablation(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第7期
- Page:
- 850-855
- Research Field:
- 医学信号处理与医学仪器
- Publishing date:
Info
- Title:
- Comparison of temperature open-loop and closed-loop feedbacks in high-voltage pulsed electric field ablation
- Author(s):
- ZHANG Ke1; 2; WANG Jie2
- 1. School of Medical Imaging, Nanjing Medical University, Nanjing 210029, China 2. Department of Interventional Radiology, Jiangsu Province Hospital, Nanjing 210029, China
- Keywords:
- Keywords: high-voltage pulsed electric field ablation temperature closed-loop
- PACS:
- R318
- DOI:
- DOI:10.3969/j.issn.1005-202X.2021.07.012
- Abstract:
- Abstract: Objective To compare the differences between temperature open-loop and closed-loop feedbacks in high-voltage pulsed electric field ablation. Methods Three different loads, namely cell suspension, 3D cell model and swine liver tissue ex vivo both in temperature open-loop group and temperature closed-loop group were exposed to high-voltage square-wave pulsed electric field with a field strength of 1.5 kV/cm, frequency of 1 Hz, pulse width of 100 μs and pulse number of 20. Moreover, a temperature closed-loop algorithm was added in closed-loop group to control pulse delivery of temperature sensor to the microcontroller. Electrode temperature, cell suspension conductivity and cell death rate, 3D cell model ablation area and pathological characteristics of swine liver tissue ablation zone after electric field ablation were quantitatively compared and qualitatively analyzed. Results The electrode temperature, cell suspension conductivity, cell death rate and 3D cell model ablation area in open-loop group after ablation were (53.2±5.5) ℃, (1.43±0.03) S/m, (86.8±5.5)% and (0.46±0.15) cm2, respectively, higher than (38.5±1.6)℃, (1.21±0.02) S/m, (73.6±10.2)% and (0.37±0.08) cm2 in closed-loop group, and the differences were statistically significant (all P<0.05). Through hematoxylin-eosin staining, coagulation necrosis caused by thermal injury was found in swine liver tissue ablation zone in open-loop group, while widening in hepatic sinusoids, cytoplasmic dehydration and loss, and nuclear accumulation were observed in closed-loop group. Conclusion Under the experimental conditions, there are differences between temperature open-loop and closed-loop feedbacks groups. The traditional temperature open-loop high-voltage pulsed electric field has a slightly stronger ablation effect than temperature closed-loop ablation, which may be related to the increase in electrical conductivity and the enhancement of the lethal effects of electric field mediated by thermal effects. Temperature closed-loop feedback can avoid thermal damage effects and achieve real non-thermal ablation.
Last Update: 2021-07-26