Effects of NIS gene transfection on NIS protein expression and iodine uptake in hepatocellular carcinoma(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2021年第10期
- Page:
- 1219-1222
- Research Field:
- 医学放射物理
- Publishing date:
Info
- Title:
- Effects of NIS gene transfection on NIS protein expression and iodine uptake in hepatocellular carcinoma
- Author(s):
- FAN Yixiang1; YI Ziwei1; HU Yulin1; ZHANG Hongjia1; LIN Meizhen1; CHEN Jiefang1; XIAO Han2
- 1. Department of Nuclear Medicine, the Fifth Affiliated Hospital of Southern Medical University, Guangzhou 510900, China 2. Department of Nuclear Medicine, Guangdong Second Provincial General Hospital, Guangzhou 510317, China
- Keywords:
- Keywords: hepatocellular carcinoma sodium/iodine symporter radioactive iodine therapy NIS gene iodine uptake apoptosis
- PACS:
- R34；R817.1
- DOI:
- DOI:10.3969/j.issn.1005-202X.2021.10.007
- Abstract:
- Abstract: Objective To explore the method of targeted radionuclide therapy for hepatocellular carcinoma. Methods The NIS gene fragment was extracted, and the recombinant plasmid pCDNA3/NIS was constructed. The recombinant plasmid was introduced into hepatocellular carcinoma HepG2 cells. The NIS protein expression in HepG2 cells at 24 h after transfection was detected by Western-Blot assay and the iodine uptake rate of the transfected cells was evaluated by 125I binding assay and the apoptosis of HepG2 cells after 125I uptake was evaluated by DAPI staining. NIS protein expression, iodine uptake rate and apoptosis rate were compared between experimental group and control group using t test. Results Protein electrophoresis showed that after NIS gene transfection, NIS protein was expressed in HepG2 cells in experimental group, and the expression intensity was significantly higher than that in control group (t=2.693,P<0.05). The iodine uptake rate (B/T%) of HepG2 cells in experimental group was (18.4±5.8)%, significantly higher than that of control group (t=36.842, P<0.05). At 24 h after 125I uptake, the number of apoptotic cells in experimental group was larger than that in control group, and the average apoptosis rate in experimental group was (19.2±5.3)%, significantly higher than that in control group (t=3.086, P<0.05). Conclusion The transfection of exogenous NIS gene can up-regulate NIS protein expression in hepatocellular carcinoma HepG2 cells, enable the cells to have the function of iodine uptake, and accelerate cell apoptosis. The study provide an experimental basis for targeted radionuclide therapy.
Last Update: 2021-10-28