Abstract

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Title:: Construction of osteoblast membrane sheet on thesurface of antibacterial titanium alloy Ti6Al4V-6Cu

Author(s):: LI Shuman1; XU Xiongcheng2; HE Mengjiao2; ZHONG Quan2; 3; CHEN Chao2; 3; WU Xiaohong2; 3; LUO Kai2; 3; 1.Department of Stomatology, Geriatric Hospital of Fujian Province, Fuzhou 350003, China 2.Laboratory of Oral Tissue Engineering, Fujian Medical University, Fuzhou 350002, China 3.Department of Periodontology, Hospital of Stomatology Affiliated to Fujian Medical University, Fuzhou 350002, China

Keywords:: antibacterial titanium alloy Ti6Al4V-6Cu metals osteoblast membrane sheet

Abstract:: Objective To study the feasibility of constructing rat jaw osteoblast membrane sheet on the surface of antibacterial titanium alloy Ti6Al4V-6Cu. Methods Osteoblasts were isolated and cultured in vitro from jaw bone of rats firstly. Osteoblast membrane sheets were then constructed on the surface of the antibacterial titanium alloy Ti6Al4V-6Cu by using vitamin C-rich medium, and with those using pure medium as the control group, the expression of alkaline phosphatase (ALP) and osteoblast-related genes ALP, type I collagen (Col-1), and bone morphogenetic protein (BMP-2) was detected and analyzed. Results Osteoblast membrane sheet can be successfully constructed on the surface of antibacterial titanium alloy Ti6Al4V-6Cu by continuous culture using vitamin C-rich medium. The osteoblast membrane sheet was composed of multiple layers of osteoblasts and rich in extracellular matrix. Compared with the control group, the ALP activity of osteoblasts and the expression of osteoblast-related genes ALP, Col-1, BMP-2 were significantly increased in the osteoblast membrane sheet group during formation. Conclusions Osteoblast membrane sheets can be successfully constructed on the surface of antibacterial titanium alloy Ti6Al4V-6Cu, so it is hopeful to combinedly apply Ti6Al4V-6Cu to guided bone regeneration.