Preparation of polypeptide-based fluorescent molecular probe for targeting lung adenocarcinoma and its application(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2020年第6期
- Page:
- 762-768
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Preparation of polypeptide-based fluorescent molecular probe for targeting lung adenocarcinoma and its application
- Author(s):
- LI Guiping; FU Zhenmin; JIANG Ying; QI Yongshuai; CHI Xiaohua; HE Yun
- Department of Nuclear Medicine, Nanfang Hospital of Southern Medical University, Guangzhou 510515, China
- Keywords:
- Keywords: Lung cancer small molecule polypeptide fluorescent molecular probe A549 cell tumor-bearing nude mice
- PACS:
- R34；R730.49
- DOI:
- DOI:10.3969/j.issn.1005-202X.2020.06.020
- Abstract:
- Abstract: Objective To explore the feasibility of realizing fluorescence imaging of lung adenocarcinoma by polypeptide-based fluorescent molecular probe which is constructed by choosing the small molecule polypeptide cNGQGEQc-L binding to integrin α3 receptor as a targeting carrier, and then connecting fluorescein FAM with cNGQGEQc-L. Methods The combination of FAM-cNGQGEQc-L with lung adenocarcinoma A549 cells was observed by an inverted fluorescence microscope. Flow cytometry was used to detect the competitive inhibition between fluorescence-labeled polypeptide and A549 cells. The change of the binding capacity of FAM-cNGQGEQc-L with A549 cells was also observed as the increasing of FAM-cNGQGEQc-L concentration. The biodistribution characteristics of fluorescence-labeled polypeptide in tumor-bearing nude mice were observed by in vivo small animal imaging. Results The inverted fluorescence microscope showed that FAM-cNGQGEQc-L could bind to A549 cells on the cell membrane and in cytoplasm. The observation by flow cytometry showed that the binding of the fluorescence-labeled polypeptide to A549 cells was characterized by specificity and saturability, and the binding could approach saturation when FAM-cNGQGEQc-L was at the concentration of 0.125 mmol/L. The in vivo imaging of tumor-bearing nude mice showed that fluorescence-labeled polypeptides could be taken up by xenograft tumors and excreted through the urinary system and the biliary system. Conclusion The results of in vitro and in vivo fluorescence experiments demonstrate that the polypeptide-based fluorescent molecular probe FAM-cNGQGEQc-L can not only bind to A549 cells and lung cancer xenograft tumors, but also specifically target lung adenocarcinoma.
Last Update: 2020-07-03