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Title:: Effects of cold atmospheric plasma on the proliferation and apoptosis of tumor cells and its mechanisms

Author(s):: WANG Tian1; 2; WANG Huanbo1; 3; AN Guangzhou1; ZHANG Junping1; 4; ZHANG Keying1; ZHOU Yan1; LI Jing5; GUO Ling1; LI Heping5; DING Guirong1; 1. Department of Radiation Protection Medicine/Faculty of Military Preventive Medicine, Air Force Medical University, Xian 710032, China 2. Department of Minimally Invasive Neurosurgery and Translational Medicine, Xian Central Hospital, Xian 710032, China 3. Cadet Company, School of Basic Medicine, Air Force Medical University, Xian 710032, China 4. Military Health Team of 61255 Troops of the Chinese Peoples Liberation Army, Houma 043000, China 5. Department of Engineering Physics, Tsinghua University, Beijing 100084, China

Keywords:: Keywords: cold atmospheric plasma tumor cell cell proliferation apoptosis oxidative stress

Abstract:: Abstract: Objective To explore the effects of cold atmospheric plasma (CAP) on the proliferation and apoptosis of human tumor cells. Methods Human glioma cells (U87) and cervical cancer cells (Hela) which were in logarithmic growth phase were inoculated in culture plate or culture dish. When the cells grew?dhering?o?he?all, they were randomly divided into control group, helium (He) group and CAP groups with different treatment durations. After different treatments, the cell viability was detected by CCK-8 method, and apoptosis was tested by flow cytometry, and the expression levels of caspase-3 protein related to apoptosis and Nrf2 protein related to oxidative stress were measured by Western blot. Results He treatment did not affect the proliferation and apoptosis of U87 cells and Hela cells. Compared with those in control group and He group, the viability of U87 cells and Hela cells in CAP group were significantly decreased, while the apoptosis rate was obviously increased. Moreover, the expression levels of caspase3 protein and Nrf2 protein in CAP group were remarkably increased, and the increase was more obvious with the longer duration of CAP treatment. Conclusion CAP can inhibit the proliferation of human tumor cells and induce apoptosis under this experimental condition, and the oxidative stress might be involved in this process.