Effects of methylation of androgen reciptor-αA gene and total homocysteinet on intracranial arterial stenosis(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2020年第3期
- Page:
- 311-316
- Research Field:
- 医学影像物理
- Publishing date:
Info
- Title:
- Effects of methylation of androgen reciptor-αA gene and total homocysteinet on intracranial arterial stenosis
- Author(s):
- HUANG Yujuan1; WAN Li2; YANG Zhihong3; SHEN Shuwen1; 4
- 1. School of Basic Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China; 2. Department of Neurology, Xi’an Daxing Hospital, Xi’an 710016, China; 3. Department of Encephalopathy, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712046, China; 4.School of Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China
- Keywords:
- Keywords: androgen receptor-αA; methylation; total homocysteinet; intracranial artery stenosis
- PACS:
- R35；R445.2
- DOI:
- DOI:10.3969/j.issn.1005-202X.2020.03.011
- Abstract:
- Abstract: Objective To discuss the relationship between intracranial arterial stenosis plaques in clinic and molecular biology under the methylation state of androgen reciptor-αA (AR-αA) gene promoter region and high-resolution magnetic resonance imaging by taking plaques of different signals in M1 segment of middle cerebral artery as example. Methods Patients with ischemic stroke were detected by high-resolution magnetic resonance imaging. A total of 69 patients with plaques in M1 segment of middle cerebral artery and neurological defect symptoms were enrolled in the study, and 30 cases in control group. Intracranial arterial plaque signals were evaluated. Methylation specific polymerase chain reaction (MSP) was used for detecting methylation state of AR-αA gene promoter region of venous blood, and fluorescence method for determining the level of serum total homocysteinet (tHcy). Results The methylation rate of AR-αA gene promoter region in the high signal or mixed signal group (n=42) was 78.6%, and that was 70.4% in iso-signal or low signal group (n=27) and 26.6% in control group (n=30). There were statistical differences in methylation rate among 3 groups (P<0.05). Meanwhile, the level of tHcy in experimental group was significantly higher than that in control group (P<0.05). The correlation coefficient r between the methylation of AR-αA gene promoter region and tHcy was 0.549. Conclusion The instability of high signal plaques in intracranial artery is increased and is positively related to tHcy, which is more likely to lead to intracranial artery stenosis.
Last Update: 2020-04-02