Abstract

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Title:: Effects of shear stress intensity on the expression of Piezo1 in vascular endothelial cells

Author(s):: ZHAO Yiwei1; REN Peile1; YU Fengxu2; DAI Hongyan3; NIE Yongmei2; 1. Department of Physiology, Collage of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China; 2. Department of Cardiovascular Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China; 3. Laboratory Centre of Mechanism, Collage of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China

Keywords:: ; Keywords: shear stress; Piezo1; endothelial cell; arteriosclerosis

Abstract:: Abstract: Objective To investigate the effects of shear stress of different intensities (0, 2.37, 4.14, 7.11, 9.47, 14.21, 17.76 dyne/cm2) on the expression of Piezo1 in vascular endothelial cells. Methods The human umbilical vein endothelial cell lines (EA.hy926) were cultured in vitro, and the shear stress of different intensities were applied on endothelial cell lines by parallel-plate flow chamber. Real-time fluorescent quantitative PCR and Western-blot analysis were used to detect the expression of Piezo1 in endothelial cell lines at gene level and protein level. Results The expression of Piezo1 was affected by shear stress intensity. The expressions of Piezo1 mRNA and proteins under a shear stress of 4.14 dyne/cm2 were significantly reduced as compared with those in control group (P<0.05). Conclusion Shear stress intensity has significant effects on the expression of Piezo1 in vascular endothelial cells, and when the shear stress intensity is 4.14 dyne/cm2, the effect is the most obvious, which indicates that Piezo1 may involve in the arteriosclerosis induced by shear stress of low intensity.