Effects of cold atmospheric plasma on coagulation function of rats(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2019年第3期
- Page:
- 351-355
- Research Field:
- 医学生物物理
- Publishing date:
Info
- Title:
- Effects of cold atmospheric plasma on coagulation function of rats
- Author(s):
- ZHANG Keying1; 6; CHEN Qiliang2; RUI Gang1; 6; WU Xiaozheng1; 6; GUO Ling1; 6; ZHANG Junping1; 6; LIU Zhiqiang4; 5; LI Heping5; DING Guirong1; 6
- 1. Department of Medical Radiation Protection, Faculty of Military Preventive Medicine, Air Force Medical University, Xi’an 710032, China; 2. The Second Squadron of Basic Medical School, Air Force Medical University, Xi’an 710032, China; 3. the 78506th Troops of Chinese People’s Liberation Army, Chengdu 610000, China; 4. College of Mechanical Engineering, North China University of Science and Technology, Tangshan 063210, China; 5. Department of Engineering Physics, Tsinghua University, Beijing 100084, China; 6.Key Laboratory of Hazard Assessment and Control in Special Operational Environment, Ministry of Education, Xi’an 710032, China
- Keywords:
- ; Keywords: cold atmospheric plasma; blood coagulation; bleeding model; rat
- PACS:
- R318
- DOI:
- DOI:10.3969/j.issn.1005-202X.2019.03.021
- Abstract:
- Abstract: Objective To investigate the effects of cold atmospheric plasma (CAP) on blood coagulation, determine the optimal parameters and explore its mechanism. Methods Firstly, the blood drops of Sprague-Dawley (SD) rats were treated with CAP with different treatment distances (1, 2 and 3 cm), different discharge voltages (3.33, 6.73 and 8.03 kV), and different treatment durations (30, 60 and 120 s) to investigate the effects of CAP with different parameters on blood coagulation and determine the optimal parameters for blood coagulation. Subsequently, 22 SD rats were randomly divided into control group (n=11) and CAP treatment group (n=11) to verify the hemostatic effect of CAP with the optimal parameters in different rat bleeding models. Finally, the mechanism of CAP to promote blood coagulation was explored by anticoagulation test. Results The results of in vitro blood test showed that CAP significantly promoted blood coagulation, which was featured with surface drying and the increasing number of dimple signs. Besides, the effect of CAP on blood coagulation was positively correlated with treatment durations and discharge voltages, but negatively correlated with treatment distances. The research on rats bleeding models showed that CAP with the optimal parameters (discharge voltage of 8.03 kV, treatment duration of 120 s, and treatment distance of 1 cm) significantly shortened the bleeding time and reduced the amount of bleeding. The results of anticoagulant test proved that CAP could significantly promote the coagulation of anticoagulant blood drops with sodium citrate. Conclusion CAP significantly shortens the time for blood coagulation. The effect of CAP on blood coagulation is closely dependent on treatment distance, discharge voltage and treatment duration, and its mechanism may be related with the conversion of fibrinogen to fibrin.
Last Update: 2019-03-26