Cranial window in mouse and its application for optical brain imaging in vivo(PDF)
《中国医学物理学杂志》[ISSN:1005-202X/CN:44-1351/R]
- Issue:
- 2017年第12期
- Page:
- 1220-1231
- Research Field:
- 医学影像物理
- Publishing date:
Info
- Title:
- Cranial window in mouse and its application for optical brain imaging in vivo
- Author(s):
- WAN Xiaofang1; WU Jinbo 2; ZHAI Peng 3; YING Ming2; ZHOU Jie1; WANG Xiaomei3; QU Junle1; XU Gaixia1
- 1. Key Laboratory of Biomedical Optical Micro-Nano Measuring and Imaging of Shenzhen, Key Laboratory of Optoelectronic Devices and System of the Ministry of Education/Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China; 2. College of Life Science, Shenzhen University, Shenzhen 518060, China; 3. Key Laboratory of Biomedical Engineering of Shenzhen, School of Medicine, Shenzhen University, Shenzhen 518060, China
- Keywords:
- ; Keywords: cranial window; in vivo brain imaging; Alzheimer’s disease; neural circuits
- PACS:
- R319
- DOI:
- DOI:10.3969/j.issn.1005-202X.2017.12.007
- Abstract:
- Abstract: Cranial window in mouse, as a kind of in vivo optical imaging aided instrument, with the advantages of in situ representation, real time observation and long-term imaging, has became one of the most important methods for the research of brain diseases and synapse growth. The cranial window can be used to visualize the leptomeningeal vessels and quantitatively analyze the flow velocity and flow regime changes of the erythrocytes in blood vessels. Moreover, combined with the fluorescent markers and optical microscopes, the cranial window can be applied for obtaining the clear images of the brain cortical tissue and the neurons. Over the past few decades, with the aid of cranial window, researchers made lots of breakthroughs on the studies of migraine, Alzheimer’s disease and the synapses. Recently, with the development of optical imaging tools, the application of cranial window had been further expanded. Herein, the history of cranial window and construction methods were introduced. We also reviewed the applications of cranial window combined with the fluorescent markers and optical microscopes on optical brain imaging (including imaging for important biological molecules in brain, cells, blood vessels, blood flow, plasticity of neural circuits and neurological functions, and research for pathogenesis and drug screening). Finally, the challenges of different cranial windows in practical application were discussed and the future applications of optical cranial window were prospected.
Last Update: 2017-12-20